Work

Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation

Year: 2015

Type: article

Abstract: EgtB from Mycobacterium thermoresistibile catalyzes O2-dependent sulfur-carbon bond formation between the side chains of Nα-trimethyl histidine and γ-glutamyl cysteine as a central step in ergothioneine biosynthesis. A single point mutation converts this enzyme into a γ-glutamyl cysteine dioxygenase with an efficiency that rivals naturally evolved thiol dioxygenases.

Source: Chemical Communications

DOI: 10.1039/c5cc07772a

Authors: Kristina V. Goncharenko, Florian P. Seebeck

Institution: University of Basel

Language: English

Topic: Metal-Catalyzed Oxygenation Mechanisms

Subfield: Inorganic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Funder: European Research Council

Award: G5536405742