Analysis of T lymphocytes infiltrating human hepatic allografts.
Year: 1987
Type: article
Abstract: Liver transplantation has become an accepted form of treatment for a variety of end-stage liver diseases. In addition to improved surgical techniques, important advances in the immunosuppressive treatment of hepatic allograft recipients have been made through the introduction of cyclosporine (Cs) and, more recently, the addition of a T cell-specific murine monoclonal antibody (MoAb) OKT3 to treat acute rejection episodes.1,2 In spite of these achievements, allograft rejection remains a major problem in liver transplantation.3,4
T lymphocytes play a major role in cell-mediated allograft rejection. During the course of alloactivation, T cells are primed against donor antigens. Alloactivated T cells then express Interleukin-2 (IL-2) receptors on their cell surface, and IL-2 is required for their further propagation. Based on this rationale, IL-2 has been used to propagate alloactivated T cells in vitro from liver allograft biopsies. Earlier studies have demonstrated the feasibility of these methodologies using IL-2 as a sole source of growth requirement. Further analysis revealed proliferative as well as cytotoxic responses of these cultured cell lines to donor HLA antigens present on donor splenocytes.5 We report results of our extended study which now includes 36 biopsies from 31 liver transplant recipients.
Source: PubMed
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2214494091