Antihyperuricemic Activity of Four Plants Annonaceae Using Hyperuricemic Rats Model and Enzyme Assay
Year: 2015
Type: article
Abstract: Objective: The aim of the research is to study the antihyperuricemic activity of Annona muricata L, Annona squamosa L, Annona reticulata L, and Stelechocarpus burahol (Bl.) Hook. F. & Th. (Annonaceae). Methods: The dried powdered leaves were extracted using ethanol by maceration method. The ethanolic extracts of four plants were administered orally to potassium oxonate-induced rats. Serum samples were collected from rats at the 1 st to 3 hr after drug administration. Enzyme assay was done by xanthine oxidase (XO). The XO inhibitory activity was measured by UV-Vis spectrophotometry, and the degree of enzyme inhibition was determined by measuring the absorbance of uric acid formation. rd Results: The result of this study revealed that all of the ethanolic extracts had a significant effect on hyperuricemic rats, and S. burahol extract decreased uric acid level was equal to level normal control. However, the antihyperuricemic activity was not parallel with XO inhibitor activity which showed weak activity with IC 50 of A. reticulata was 171.73 µg/ml, while IC 50 2 of A. muricata, A. squamosa, and S. burahol more than 200 µg/ml. Conclusion: The ethanolic extract of S. burahol was more potential than three other extracts in decreasing in uric acid levels. The results scientifically confirmed that S. burahol can be developed as potential antihyperuricemic agents. Keywords: Antihyperuricemia, Annona muricata, Annona squamosa, Annona reticulata, Stelechocarpus burahol, Potassium oxonate, Xanthine oxidase.
Institution: Universitas Setia Budi
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2216008481