Year: 2009
Type: article
Abstract: The behavioral and physiological effects of lipopolysaccharide induced mastitis were examined in lactating Holstein cows.Twenty cows were assigned to five blocks of four cows grouped by parity and stage of lactation.The experimental design for this study was a 2 X 2 factorial arrangement.Cows within the blocks were randomly assigned to one of four treatments: 1) intramammary infusion with 25 µg lipopolysaccharide and intravenous flunixin meglumine; 2) intramammary infusion with 25 µg lipopolysaccharide and intravenous PBS; 3) intramammary infusion with PBS and intravenous flunixin meglumine; and 4) intramammary infusion PBS and intravenous with PBS.Uninfected mammary quarters were infused 3 h post milking.Intravenous treatments were given 7 h post milking.Experimental cows were under continuous video monitoring during the study.Cows receiving the lipopolysaccharide treatment had higher mean peak quarter milk somatic cell counts, rectal temperatures, concentrations of milk amyloid and serum cortisol during the first 12 h after infusion compared with intramammary saline treated cows.Lipopolysaccharide treated cows spent a reduced percentage of the first 24 h after challenge eating and cud chewing compared with saline infused cows.Rumen contractions were reduced in lipopolysaccharide infused cows compared with saline infused cows at sample times corresponding with peak rectal temperatures.Flunixin treatment 4 h after intramammary challenge mitigated the clinical ix STATISTICS. . . . . . . . . . .
Author: Jennifer Laura Zimov
Institution: The Ohio State University
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: green
Funder: Ohio State University
OpenAlex ID: W2221395847