Work

Fabrication and application of heterogeneous printed mouse phantoms for whole animal optical imaging

Year: 2016

Type: article

Abstract: This work demonstrates the usefulness of 3D printing for optical imaging applications. Progress in developing optical imaging for biomedical applications requires customizable and often complex objects for testing and evaluation. There is therefore high demand for what have become known as tissue-simulating "phantoms." We present a new optical phantom fabricated using inexpensive 3D printing methods with multiple materials, allowing for the placement of complex inhomogeneities in complex or anatomically realistic geometries, as opposed to previous phantoms, which were limited to simple shapes formed by molds or machining. We use diffuse optical imaging to reconstruct optical parameters in 3D space within a printed mouse to show the applicability of the phantoms for developing whole animal optical imaging methods. This phantom fabrication approach is versatile, can be applied to optical imaging methods besides diffusive imaging, and can be used in the calibration of live animal imaging data.

Source: Applied Optics

DOI: 10.1364/ao.55.000280

Authors: Brian Z. Bentz, Anmol V. Chavan, Dergan Lin, Esther H. R. Tsai, Kevin J. Webb

Institution: Purdue University West Lafayette

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Cancer Institute

Awards: Towards Deep Tissue Imaging of Fluorescence Resonance Energy Transfer Parameters, CIF: Small: Fast In Vivo Optical Imaging in the Heavily and Weakly Scattering Regimes, Optical Imaging of Targeted Anti-Cancer Drug Delivery Kinetics