Work

Cyclic dinucleotide detection with riboswitch–G-quadruplex hybrid

Year: 2015

Type: article

Abstract: A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split G-quadruplex for this conditional peroxidase-mimicking riboswitch.

Source: Molecular BioSystems

DOI: 10.1039/c5mb00751h

Authors: Genichiro Tsuji, Herman O Sintim

Institutions: University of Maryland, College Park, Purdue University West Lafayette

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: National Science Foundation, Camille and Henry Dreyfus Foundation, Division of Chemistry

Awards: New Receptors for signaling bacterial nucleotides, G2801098644