The long noncoding RNA landscape in hypoxic and inflammatory renal epithelial injury
Year: 2015
Type: article
Abstract: Long noncoding RNAs (lncRNAs) are emerging as key species-specific regulators of cellular and disease processes. To identify potential lncRNAs relevant to acute and chronic renal epithelial injury, we performed unbiased whole transcriptome profiling of human proximal tubular epithelial cells (PTECs) in hypoxic and inflammatory conditions. RNA sequencing revealed that the protein-coding and noncoding transcriptomic landscape differed between hypoxia-stimulated and cytokine-stimulated human PTECs. Hypoxia- and inflammation-modulated lncRNAs were prioritized for focused followup according to their degree of induction by these stress stimuli, their expression in human kidney tissue, and whether exposure of human PTECs to plasma of critically ill sepsis patients with acute kidney injury modulated their expression. For three lncRNAs (MIR210HG, linc-ATP13A4-8, and linc-KIAA1737-2) that fulfilled our criteria, we validated their expression patterns, examined their loci for conservation and synteny, and defined their associated epigenetic marks. The lncRNA landscape characterized here provides insights into novel transcriptomic variations in the renal epithelial cell response to hypoxic and inflammatory stress.
Institution: University of Pennsylvania
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
Awards: Mentored Patient Oriented Research in Cardiometabolic Disease, Epigenetics of Chronic Kidney Disease, Elucidation of Tissue-Specific Transcriptomic Profiles in Cardio-metabolic Disease, Epigenetic drivers and biomarkers of diabetic kidney disease, G6296410657, Institutional Clinical and Translational Science Award, Acute Adipose Inflammation as a Contributor to Acute Kidney Injury After Trauma, Statistical Methods for Transcriptome Profiling Using RNA Sequencing, Training in Renal Research
OpenAlex ID: W2226811668