Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle
Year: 2016
Type: article
Study design: Observational Study
Abstract: Calorie restriction (CR) retards aging, acts as a hormetic intervention, and increases serum corticosterone and HSP70 expression in rodents. However, less is known regarding the effects of CR on these factors in humans. Serum cortisol and molecular chaperones and autophagic proteins were measured in the skeletal muscle of subjects on CR diets for 3-15 years and in control volunteers. Serum cortisol was higher in the CR group than in age-matched sedentary and endurance athlete groups (15.6 ± 4.6 ng/dl versus 12.3 ± 3.9 ng/dl and 11.2 ± 2.7 ng/dl, respectively; p ≤ 0.001). HSP70, Grp78, beclin-1, and LC3 mRNA and/or protein levels were higher in the skeletal muscle of the CR group compared to controls. Our data indicate that CR in humans is associated with sustained rises in serum cortisol, reduced inflammation, and increases in key molecular chaperones and autophagic mediators involved in cellular protein quality control and removal of dysfunctional proteins and organelles.
Source: Cell Reports
Institutions: Harvard University, AREA Science Park, Cluster in Biomedicine, Washington University in St. Louis, Sapienza University of Rome, University of Padua, University of Verona, Università Campus Bio-Medico, Michael E. DeBakey VA Medical Center, Baylor College of Medicine, CEINGE Biotecnologie Avanzate Franco Salvatore (Italy), University of Brescia
Language: English
Subfield: Aging
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,620
Awards: G1074184956, G1513996379, G2202404319, G413336477, G4467312596, The role of immunometabolic pathways in atherosclerosis, Washington University Institute of Clinical and Translational Sciences, Inflammatory and stress signaling networks in metabolic disease, Washington University Institute of Clinical and Translational Sciences (UL1)
OpenAlex ID: W2228223409