Work

Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes

Year: 1979

Type: article

Abstract: Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used.

Source: Biochemical Journal

DOI: 10.1042/bj1840277

Authors: D M Wallace, R Jagus, C R Benzie, J E Kay

Institutions: National Institutes of Health, University of Sussex, National Heart, Lung, and Blood Institute, National Cancer Institute

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Medical Research Council

OpenAlex ID: W2235267322

FWCI: 1.066

Cites: 28

Cited by: 14

Related to: 10