Resveratrol attenuates β-amyloid-induced oxidative stress in astrocytes.
Year: 2010
Type: article
Abstract: Objective To study the effect of resveratrol on β-amyloid-induced oxidative injury in cultured astrocytes and the protective effects of resveratrol on the central nervous system. Methods Astrocytes were treated with various concentrations of resveratrol for 12 h and then exposed to Aβ. Cell viability was detected by CCK-8 assay. ROS was measured using dihydroethidium (DHE) and the fluorescence was measured by multilabel counter. The level of lactate dehydrogenase (LDH) leakage was examined after injury. Results 10,100 μmol / L resveratrol pretreatment was found to significantly increase the cell viability. DHE intensity was decreased when the cells were pretreated with 10,100 μmol / L of resveratrol,whereas 1 μmol / L of resveratrol increased the DHE intensity. LDH leakage was significantly increased after Aβ treatment of the astrocytes,and the LDH leakage was inhibited after resveratrol (10,100 μmol / L) pretreatment. Conclusion Resveratrol could increase the cell viability and inhibit the release of LDH leakage,and β-amyloid-induced oxidative injury can be decreased with resveratrol pretreatment.
Source: Journal of Tropical Medicine
Language: English
Subfield: Geriatrics and Gerontology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,630
OpenAlex ID: W2235536635