Molecular identification of the dominant microbiota and their spoilage potential of Crangon crangon and Raja sp.
Year: 2012
Type: dissertation
Abstract: This study points out the limitations of several general growth media frequently used in seafood research by a systematic identification of the microorganisms on fish samples during ice storage unable to grow on those media. Aerobic psychrotolerant count (APC), replication on various general media and total cultivable microbiological community PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analysis revealed that many potential spoilage microorganisms were overlooked. Those microorganisms overlooked by using only one single growth medium were identified by partial 16S rRNA gene and gyrB gene sequencing. Members of the genera Shewanella, Vibrio, Aliivibrio, Photobacterium, Pseudoalteromonas and Psychrobacter, including Photobacterium phosphoreum, Shewanella baltica and Pseudomonas fluorescens were unable to grow on PCA. APC analysis also confirmed that on plate count agar (PCA) the enumeration of the microbiota was underestimated. Although Long and Hammer agar (LH) and marine agar (MA) obtained the best quantitative (APC analysis) and qualitative (replication and PCR-DGGE analyses) results for fish quality analysis, analysts have to keep in mind that some species were also unable to grow on those media, such as Pseudomonas fragi and Acinetobacter sp.
Author: Katrien Broekaert
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
SDGs: Life below water, Zero hunger
Open Access status: green
OpenAlex ID: W2236340042