Year: 2016
Type: article
Abstract: Polymers have been used extensively taking forms as scaffolds, patterned surface and nanoparticle for regenerative medicine applications. Angiogenesis is an essential process for successful tissue regeneration, and endothelial cell–cell interaction plays a pivotal role in regulating their tight junction formation, a hallmark of angiogenesis. Though continuous progress has been made, strategies to promote angiogenesis still rely on small molecule delivery or nuanced scaffold fabrication. As such, the recent paradigm shift from top-down to bottom-up approaches in tissue engineering necessitates development of polymer-based modular engineering tools to control angiogenesis. Here, we developed cationic nanocylinders (NCs) as inducers of cell–cell interaction and investigated their effect on angiogenic activities of human umbilical vein endothelial cells (HUVECs) in vitro. Electrospun poly (l-lactic acid) (PLLA) fibers were aminolyzed to generate positively charged NCs. The aninolyzation time was changed to produce two different aspect ratios of NCs. When HUVECs were treated with NCs, the electrostatic interaction of cationic NCs with negatively charged plasma membranes promoted migration, permeability and tubulogenesis of HUVECs compared to no treatment. This effect was more profound when the higher aspect ratio NC was used. The results indicate these NCs can be used as a new tool for the bottom-up approach to promote angiogenesis.
Source: Polymers
DOI: 10.3390/polym8010015
Authors: Jung Lee, Daniel Balikov, Jae Yang, Ki Kim, Hun Park, Jeong Kim, Il Kwon, Leon Bellan, Hak-Joon Sung
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,258
Funders: National Science Foundation, Vanderbilt University, National Institutes of Health, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt University, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering, Division of Materials Research, National Center for Research Resources, Division of Chemical, Bioengineering, Environmental, and Transport Systems, National Center for Advancing Translational Sciences
Awards: G1182793083, Tennessee Solar Conversion and Storage using Outreach, Research and Education (TN-SCORE), CAREER: Oxidative Stress - Responsive Shape Memory Vascular Patch, Medical Scientist Training Program, A New Class of Biomaterials Programmed to Regulate Host Body Responses, G4425699080, G4858526758, Thermosensitive injectable polymer-based stem cell therapy for limb ischemia, G560889077, G563474107, G5679716143, G5868276417, The Vanderbilt Institute for Clinical and Translational Research (VICTR) UL1, G6671297155, G6711204355, G6918005705, G7053408464, G7307553525, The Vanderbilt Institute for Clinical and Translational Research (VICTR), Neurovascular Unit on a Chip: Chemical Communication, Drug and Toxin Responses, G8521655310, G9000033427, G9000300101
OpenAlex ID: W2236774779