A Comparative Study of Three Non-invasive Systems for Measurement of Hemoglobin with HemoCue System Having Coulter LH750 as Reference Value
Year: 2015
Type: article
Abstract: Context: The measurement of hemoglobin (Hb) is a priority in blood donor's selection.In recent years non-invasive methods have appeared based on spectrophotometry and spectroscopy for Hb measurement, avoiding the inconvenience of the fingerstick systems used in the developed countries.Objective: To analyze three non-invasive methods: NBM200 (2L0177 probe) which uses spectroscopy occlusion, Haemospect (01.81.191 version) with spectroscopic measurement and quantitative evaluation and Pronto7 TM (2.3.16version) which uses spectrophotometry with multiple wavelengths, and to compare these methods with the HemoCue 301 (invasive fingerstick system) and a venous Hb predonation sample analyzed in a hematology analyzer (Coulter LH 750) as a reference value.Design: A prospective study was performed in 2014 with 159 blood donors (102 men and 57 women).Operators who used four methods filled out a questionaire about the usability.The results were entered into a database and a statistical analysis was performed, estimating correlation index and Blatman Almant plot.We analyzed rejected falsely and accepted falsely results with the Coulter as the reference standard.Results: With HemoCue 95.6% of donors were properly accepted, 91.82% with Haemospect and NBM200 and 89.94% with Pronto7.Blood donors improperly accepted reached the 0.63% with HemoCue, 1.26% with Haemospect and NBM2000 and 2.52% with Pronto7, and 3.7% were wrongly excluded with HemoCue, 5.66% with NBM200, 6,62% with Haemospect and 8,81% with Pronto7.Correlation index was better between HemoCue and Coulter (0.859) than the other systems (Haemospect 0.416; Pronto7 0.618; NBM200 0.648). Conclusion:The value of hemoglobin with HemoCue shows the best correlation with the LH750, being lower for the rest, although acceptable, especially with P7 and NBM200.But the three devices could be used as screening test for blood.Any device is superior to any other and we should analyze its cutoff to avoid the high percentage of falsely rejected.As for handling, the 4 systems meet expectations
Author: Angel J Pajares
Institution: Complejo Hospitalario Universitario de Toledo
Language: English
Subfield: Management of Technology and Innovation
Domain: Social Sciences
Open Access status: bronze
OpenAlex ID: W2241667539