Direct Amino Acid Uptake by Plants related to Grassland Diversity - methodological and ecological Investigations
Year: 2009
Type: dissertation
Abstract: Direct plant uptake of organic nitrogen (N) is often studied using the dual labelling approach ( 15 N+ 13 C or 15 N+ 14 C).However, the method might be hampered by uptake of labelled inorganic carbon (C) produced by mineralization of labelled organic compounds.Here we report the results from a triple labelling experiment ( 15 N+ 13 C+ 14 C) investigating whether root uptake of labelled inorganic C can bias the results obtained in studies of organic N uptake using dual labelled amino acids ( 15 N, 13 C).In a rhizosphere tube experiment we investigated 13 C and 14 C uptake by maize either supplied with labelled glycine or CO 3 2-, but found no differences in uptake rates between these C-sources.The uptake of inorganic C to the shoot tissue was higher for maize grown in full light compared to shading, which indicates a passive uptake of inorganic C with water.We conclude that uptake of inorganic C produced by mineralization of amino acids can significantly bias the interpretations of organic N uptake studies using dual labelling.
Author: Leopold Sauheitl
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2248669021