Year: 2011
Type: article
Abstract: The Tol protein system is a set of proteins found between the cytoplasmic and outer membranes of Gram negative bacteria.The central component of this system is the protein TolA.The exact physiological function of both the protein system and TolA specifically remains to be determined; however data suggest a role in maintaining the integrity of the outer membrane.In addition to this hypothesized physiologic role, the Tol system has been shown to be parasitized by both filamentous bacteriophage and the toxic colicin proteins.The TolA protein is believed to facilitate the translocation of the phage and colicins across the periplasmic space.The present study characterizes phylogenetically conserved features of the TolA protein that contribute to sensitivity to group A colicins.This study provides insight into the molecular features of the protein-protein interactions involved in the translocation of group A colicins.Table of contents INTRODUCTION……………………………………………………………………….1 The Tol system…………………………………………………………………....2 Colicins…………………………………………………………………………...7 Colicin/Tol relationship…………………………………………………………..9 Specific aims……………………………………………………………………...10 MATERIALS and METHODS…………………………………………………………..12 Media……………………………………………………………………………..12 Bacterial strains and plasmids…………………………………………………….12Plasmid construction…………………………………………………………….
Author: Dana M. Zook
Institution: Bowling Green State University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2248855098