Work

Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

Year: 2015

Type: article

Abstract: Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.

Source: Nature Communications

DOI: 10.1038/ncomms8276

Authors: Kai Wang, Wenzhi Sun, Christopher T. Richie, Brandon K. Harvey, Eric Betzig, Na Ji

Institutions: Howard Hughes Medical Institute, Janelia Research Campus, National Institute on Drug Abuse

Language: English

Topic: Adaptive optics and wavefront sensing

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $6,990

Funders: Howard Hughes Medical Institute, National Institutes of Health