N-BLR, a primate-specific non-coding transcript, modulates the epithelial-to-mesenchymal transition and leads to colorectal cancer invasion and migration
Year: 2014
Type: preprint
Abstract: Non-coding RNAs have been commanding increasingly greater attention in recent years as the few that have been functionalized to date play important roles in key cellular processes. Here we show that N-BLR, a ~900 bp non-coding RNA, modulates the epithelial-to-mesenchymal transition, increases colorectal cancer invasion, and functions as a migration enabler by affecting the expression of ZEB1 and E-cadherin. In patients with colorectal cancer, N-BLR expression associates with tumor stage and invasion potential. As N-BLR contains several instances of a category of DNA motifs known as pyknons, we also designed a custom-made array to investigate the possibility that other pyknon loci may be transcribed. For several of the loci probed by the array we found that the corresponding pyknons are differentially expressed between cancer and normal tissue samples. Taken together the data suggest that a systematic study of other pyknon-containing non-coding RNAs like N-BLR may be warranted in the context of colorectal cancer.
DOI: 10.1101/004796
Authors: Isidore Rigoutsos, Sang Kil Lee, Su Youn Nam, Tina Catela Ivkovic, Martin Pichler, Simona Rossi, Peter Clark, Jing Yi, Hui Ling, Masayoshi Shimizu, Roxana Simona Redis, Maitri Yogen Shah, Xinna Zhang, Eun Jung Jung, Aristotelis Tsirigos, Li Huang, Jana Ferdin, Roberta Gafa, Riccardo Spizzo, Milena Nicoloso, Maryam Shariati, Aida Tiron, Jen Jen Yeh, Raul Teruel, Sonia Melo, Lianchun Xiao, Elsa Renee Flores, Massimo Negrini, Menashe Bar Eli, Sendurai Mani, Chang-Gong Liu, Ioana Berindan-Neagoe, Manel Esteller, Michael Keating, Giovanni Lanza, George Calin
Institutions: Thomas Jefferson University, The University of Texas MD Anderson Cancer Center, Yonsei University, Yonsei University Health System, National Cancer Center, National Cancer Center Hospital, Ruđer Bošković Institute, SIB Swiss Institute of Bioinformatics, IBM Research - Thomas J. Watson Research Center, University of Ferrara, Centro di Riferimento Oncologico, University of North Carolina at Chapel Hill, UNC Lineberger Comprehensive Cancer Center, Centro Regional de Hemodonación, Universidad de Murcia, Institut d'Investigació Biomédica de Bellvitge
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
Awards: G1573574303, G1852139970, G2169464185, M D Anderson Cancer Center Head & Neck SPORE, G2947552126, G3027680094, G4660434062, Targeting Ras-Ral GEF-Ral Effector Signaling for Pancreatic Cancer Treatment, Protein-coding and non-coding RNA biomarkers for early detection of CLL, G565305668, G5974234380, G6344965762, G6526681941, G7905850378, G8817362204, G9000038294, G9000048072, G9000209611, G9000223477, G9000339540, G91461127
OpenAlex ID: W2254748985