Year: 2011
Type: book-chapter
Abstract: Heat shock-mediated delayed protection against myocardial and cerebral ischemia has been attributed to induction of heat shock proteins (HSP), especially HSP70, in myocytes and neurons.However, heat shock preconditioning only induces HSP70 in endothelium.The purpose of this study was to examine the effect of heat shock on endothelium-dependent vasomotor tone.Isolated mouse aortas were heated to 43.5ºC for 15 min and returned to 37ºC for 18 h.They were then mounted in a wire myograph to assess vasomotor responses to vasoconstrictors and vasodilatators.Intact heat shocked aortas showed reduction in phenylephrine-or 5-hydroxytryptamine-induced vasoconstriction, while no difference in vascular reactivity was detected in heat shocked endothelium-denuded aortas.Reduced vasoconstriction in heat shocked aortas was restored to control level by incubation with L-NAME, a nitric oxide synthase (NOS) inhibitor, but not by geldanamycin, a HSP90 inhibitor.Furthermore, the effect of heat shock was not observed in aortas from eNOS null mice, suggesting that vasomotor responsiveness reduced by heat shock was related to increased basal eNOS activity.Immunoblot of lysates from heat shocked aortas did not show upregulation of eNOS or other NOS isoforms, while showing robust expression of HSP70; immunoprecipitation using eNOS polyclonal or monoclonal antibodies demonstrated HSP70 interaction with eNOS after heat shock.In enzymatic assay
Language: English
Subfield: Physiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2256477671