Comparative functional analysis of two alpha-glucosidases, Family 31 Glycoside Hydrolases from the human gut symbiont Bacteroides thetaiotaomicron
Year: 2012
Type: dissertation
Abstract: The human gut is home to a significant number of microorganisms including the dominant symbiont Bacteroides thetaiotaomicron. This microbe is predicted to possess an array of glycoside hydrolases, majority of which are involved in starch utilization. Presented here is a comparative functional analysis of two alpha-glucosidases, Family 31 Glycoside Hydrolases from Bacteroides thetaiotaomicron. Enzymatic kinetics revealed these enzymes both preferentially cleave alpha-(1,6) linkage in comparison to the predicted alpha-(1,4) and favour maltose derived substrates of longer length.
Source: UWSpace (University of Waterloo)
Author: Marcia M. Chaudet
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2257637701