Disclosing the caffeine dose to be administered that restores insulin sensitivity in prediabetes animal
Year: 2014
Type: dissertation
Abstract: Type 2 diabetes mellitus (T2DM) is a chronic disease affecting millions of individuals, contributing to significant morbidity and mortality worldwide. Caffeine is the most widely consumed psychoactive substance in the world and recently several epidemiological studies described beneficial effects of chronic coffee intake on T2DM and metabolic syndrome. Our group has shown that chronic caffeine intake (1g/l) prevents the development of insulin resistance and hypertension in diet-induced insulin resistante rats. Therefore, the main objective of this work was to investigate the therapeutic dose of caffeine that restores insulin sensitivity in a prediabetic animal model. Experiments were performed in Wistar rats. The prediabetic animal model used was the high sucrose (HSu) model, which is obtained by submitting the animals to 35% of sucrose in drinking water during 28 days. The effect of chronic caffeine (0.5, 0.75 and 1g/l) was tested in control rats and in HSu model during 12 weeks assessing: insulin sensitivity, basal glycemia, glucose tolerance, adipose tissue mass, Glut4 transporters and nitric oxide (NO) content in skeletal muscle and in the liver. We have seen that chronic caffeine intake restores insulin sensitivity and glucose tolerance in HSu rats, being the latency time inversely correlated with caffeine concentration. Also, HSu diet did not change weight gain comparing with controls but increased fat mass. Caffeine intake did not alter weight gain/day and fat mass. Caffeine (1g/l) restores Glut4 expression levels in skeletal muscle in HSu animals. NO levels decrease in skeletal muscle in HSu animals, but not in the liver, and caffeine did not modify these levels. These results suggest that caffeine can be used as a therapeutical tool for the treatment of prediabetes and prevention of T2DM.
Language: English
Topic: Coffee research and impacts
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2258488772