Keratin Hydrogels for Antibiotic Delivery in Wound Healing Applications: Development of Hplc Methods to Characterize Release
Year: 2014
Type: article
Abstract: In the United States, 450,000 burn injuries receive medical treatment per year 1 with approximately 2% experiencing wound infection 2 .Current clinical paradigms such as Epicel, Apligraf, and Alloderm may provide wound coverage and promote wound healing, but do not incorporate antibiotics to fight microbial infection.Thus, dressings or materials that can promote healing while preventing infection are an important unmet clinical need.One possible solution is a biomaterial loaded with antibiotics.Off-the-shelf use of hydrogels made from keratin, collagen, and fibronectin proteins are attractive because of their physiochemical capacity to facilitate skin healing by promoting appropriate cellular responses.This thesis focuses on keratin hydrogels due to their ability to resist proteolytic degradation, promote vascularization, and support cellular ingrowth.The principal hypothesis of these experiments is that antibiotic loaded keratin hydrogels promote sustained release in a manner correlated with their degradation.To investigate this hypothesis, this thesis developed high performance liquid chromatography (HPLC) methods for detection of antibiotics in the presence of keratin proteins.Specifically, the results of this study helped to quantify antibiotic release from keratin hydrogels, and to characterize previous results indicating the sustained release of three specific antibiotics, cephazolin, ciprofloxacin, and neomycin.The results of these studies demonstrate the suitability of keratin hydrogels for antibiotic release and support conducting pre-clinical animal testing as well as possible FDA submissions for future human trials.
Author: Rachael Catherine Hall
Institution: Miami University
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2259419838