Year: 2013
Type: article
Abstract: With formaldehyde as the major source of endogenous N⁶-formyllysine protein adducts, we quantified endogenous and exogenous N⁶-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [¹³C²H₂]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N⁶-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N⁶-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts.
Source: Chemical Research in Toxicology
DOI: 10.1021/tx400320u
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: Elucidating Risks: From Exposure and Mechanism to Outcome, MIT Center for Environmental Health Sciences, DNA Ocidation Products and Endogenous DNA Adducts, Endogenous Nitrite Carcinogenesis in Man, Chemistry and Biology of Deoxyribose Oxidation in DNA, UNC Center for Environmental Health and Susceptibility, Pre- and Postdoctoral Training in Toxicology
OpenAlex ID: W2318602160