Work

Effect of methylation on the side-chain pKavalue of arginine

Year: 2015

Type: article

Abstract: Arginine methylation is important in biological systems. Recent studies link the deregulation of protein arginine methyltransferases with certain cancers. To assess the impact of methylation on interaction with other biomolecules, the pKa values of methylated arginine variants were determined using NMR data. The pKa values of monomethylated, symmetrically dimethylated, and asymmetrically dimethylated arginine are similar to the unmodified arginine (14.2 ± 0.4). Although the pKa value has not been significantly affected by methylation, consequences of methylation include changes in charge distribution and steric effects, suggesting alternative mechanisms for recognition.

Source: Protein Science

DOI: 10.1002/pro.2838

Authors: Marina Evich, Ekaterina Stroeva, Yujun George Zheng, Markus W. Germann

Institutions: Georgia State University, University of Georgia

Language: English

Topic: Cancer-related gene regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Georgia Cancer Coalition, Georgia State University, National Institute of General Medical Sciences

Award: Chemical Approaches to Protein Arginine Methylation