Work

Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition.

Year: 2014

Type: article

Abstract: OBJECTIVE: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia. METHODS: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR. RESULTS: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05). CONCLUSION: After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.

Source: Europe PMC (PubMed Central)

Authors: Zhang-Hua Li, Wen Liao, Qiang Zhao, Tang Huan, Pan Feng, Xia Wei, Yang Yi, Ning-Sheng Shao

Institutions: Renmin Hospital of Wuhan University, Hubei University of Chinese Medicine, Academy of Military Medical Sciences, Wuhan Sports University

Language: English

Topic: Mesenchymal stem cell research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2264474535

FWCI: 0.897

Cites: 30

Cited by: 14

Related to: 10