Year: 2015
Type: article
Abstract: Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.
Source: Scientific Reports
DOI: 10.1038/srep15915
Institutions: University of Oxford, Centre for Human Genetics
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,784
Awards: Establishing The Edinburgh Super-Resolution Imaging Consortium (ESRIC), Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging, MICA: Nanoscopy Oxford (NanO): Novel Super-Resolution Imaging Applied to Biomedical Sciences, Wellcome Trust Technology Platform, Advanced Microscopy for Chromosome and RNA Dynamics.
OpenAlex ID: W2269415927