Work

Expression of p-CREB and activity-dependent miR-132 in temporal lobe epilepsy.

Year: 2014

Type: article

Abstract: OBJECTIVE: The mechanisms underlying the pathogenesis of intractable epilepsy (IE) are still unclear. This study aimed to investigate the role of phosphorylation of Cyclase Response Element Binding Protein (p-CREB)/microRNA-132 (miR-132) signal pathway in the epileptogenesis. METHODS: Temporal lobe epilepsy (TLE) was induced in rodents, and the brain tissues were collected. P-CREB expression was detected by Western blot assay and immunochemistry at 6 h, 24 h, 3 d, 7 d, 14 d, 30 d and 60 d after induced status epilepsy (SE), respectively, and in patients with TLE. The expression of miR-132 was detected by RT-PCR. RESULTS: The p-CREB and miR-132 were highly expressed in both rats and patients with TLE as compared to controls. CONCLUSIONS: The expression of p-CREB and miR-132 increases in epilepsy rats and patients, suggesting a possible pathogenetic role of p-CREB and miR-132 in TLE via modulating the dendritic plasticity.

Source: Europe PMC (PubMed Central)

Authors: Jing Guo, Heng Wang, Qian Wang, Yangmei Chen, Shengli Chen

Institutions: Second Affiliated Hospital of Chongqing Medical University, Chongqing Three Gorges Central Hospital

Language: English

Topic: Epilepsy research and treatment

Subfield: Clinical Neurology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2269843685

FWCI: 3.873

Cites: 36

Cited by: 37

Related to: 10