Year: 2015
Type: dissertation
Abstract: Protein design is still in its infancy, yet there have been many impressive examples of success in designing proteins to fold into a predictable structure [6,4], to catalyse enzymatic reactions [10,13], or to bind a specific protein [16], DNA sequence [17, 11], or small molecule target [8].Each of these successes in the field is a major milestone, but protein design still lacks a generalized solution for reliably repeating these successes on future targets.The design of proteins capable of binding small molecules is particularly challenging due to the necessity to accurately understand and computationally model atomic scale physiochemical principles.We work towards this goal because being able to reliably design small molecule binders would allow for faster, and more efficient creation of detection elements for biosensors, sequestration proteins to aid in dialysis, and orthogonal binding tags for use in biotechnology applications.Even a modest advantage gained through computational design would allow for faster results when using more traditional directed evolution search methods.Since control of molecular specificity at the atomic level is essential
Author: Austin L. Day
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2271254752