Work

Generalized single-particle cryo-EM – a historical perspective

Year: 2015

Type: review

Abstract: This is a brief account of the earlier history of single-particle cryo-EM of biological molecules lacking internal symmetry, which goes back to the mid-seventies. The emphasis of this review is on the mathematical concepts and computational approaches. It is written as the field experiences a turning point in the wake of the introduction of digital cameras capable of single electron counting, and near-atomic resolution can be reached even for smaller molecules.

Source: Microscopy

DOI: 10.1093/jmicro/dfv358

Author: Joachim Frank

Institutions: Howard Hughes Medical Institute, Columbia University

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences

Award: Structural Analysis of Macromolecular Assemblies