The Effects of Carbon Dioxide Enrichment, Natural Ventilation, and Light Intensity on Growth, Photosynthesis, and Transpiration of Cauliflower Plantlets Cultured in vitro Photoautotrophically and Photomixotrophically
Year: 1998
Type: article
Abstract: The effects of natural ventilation and CO 2 enrichment during the rooting stage on the growth and the rates of photosynthesis and transpiration of in vitro cauliflower ( Brassica oleracea L.) plantlets were investigated. In vitro plantlets were established in airtight or ventilated vessels with or without CO 2 supplied (≈1200 μg·L -1 ) through gas permeable films attached to the vessel's cap for 15 days before transplanting ex vitro. Leaves generated in vitro in ventilated vessels had a higher photosynthetic rate than those produced in airtight vessels, which lead to greater leaf expansion and shoot and root dry matter accumulation during in vitro culture and acclimatization. Enhanced photosynthesis in leaves of ventilated plantlets was positively correlated with chlorophyll content. Increasing photosynthetically active radiation from 70 to 200 μmol·m -2 ·s -1 enhanced the growth of in vitro plantlets under ventilated conditions but it depressed photosynthesis of the leaves grown photomixotrophically with sugar and CO 2 enrichment which might be due to the feedback inhibition caused by marked accumulations of sucrose and starch. Higher CO 2 levels during in vitro culture enhanced photosynthesis under photoautotrophic conditions, but inhibited it under photomixotrophic conditions. Fifteen days after transplanting ex vitro, high photosynthetic ability and stomatal resistance to transpiratory water loss of ventilated plantlets in vitro had important contributions to rooting and acclimatization. Our findings show that the ventilated culture is effective for accelerating photoautotrophic growth of plantlets by increasing photosynthesis, suggesting that, especially for plantlets growing in vitro without sugar, CO 2 enrichment may be necessary to enhance photosynthetic ability.
Authors: Michio Kanechi, Masakatsu Ochi, Michiko Abe, Noboru Inagaki, Susumu Maekawa
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,500
OpenAlex ID: W2272511148