Work

Rapid isolation of high molecular weight plant DNA

Year: 1980

Type: article

Abstract: A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.

Source: Nucleic Acids Research

DOI: 10.1093/nar/8.19.4321

Authors: M.G. Murray, W.F. Thompson

Institutions: Carnegie Institution for Science, Carnegie Department of Plant Biology

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2082091267

FWCI: 1.811

Cites: 3

Cited by: 12,590

Related to: 10