Year: 2008
Type: dissertation
Abstract: In order to reconstruct esophagus due to congenital or acquired esophageal diseases, a number of materials and techniques in tissue engineering have been developed to produce biocompatible and biodegradable scaffolds to support esophageal regeneration.Recently, acellular extracellular matrix (ECM) prepared by cell removal from native tissues (a novel technique called "decellularization") has shown promising results in regeneration of tissues such as skin, blood vessels and gastrointestinal tracts.Many decellularization methods have been developed but the effects of the methods on the resulting decellularized ECMs have not been fully elucidated.The aim of this project is to study two different decellularization protocols for producing decellularized porcine esophageal ECMs.In this study, protocols from University of Washington ("UW protocol") and from Professor Stephen Badylak ("SB protocol") were employed and compared.UW protocol used Triton X-IOO, Deoxyribonuclease I and Ribonuclease A (DNAse/RNAse), and.sodium docedyl sulfate (SDS) for cell removal while SB protocol employed mechanical scraping followed by peracetic acid.Scanning electron micrographs showed that both protocols produced highly porous matrices.Porous network produced by UW protocol was more regular which resembled the native submucosa whereas SB protocol resulted in a rather random and broken structure, probably due to mechanical damage.From the histological results of decellularized
DOI: 10.32657/10356/41429
Author: Yun Chen
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Funder: Nanyang Technological University
OpenAlex ID: W2272740639