Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy
Year: 2016
Type: article
Abstract: Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160 μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.
Source: Optics Letters
DOI: 10.1364/ol.41.000855
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Awards: Vision Sciences Training Grant, G1731231054, Plasmonic devices for controlling light at the nanoscale, G2846817611, Quantum Optical and Atomic Interferometry, REU Site: Interdisciplinary Research Experience in Computational Sciences, Functional consequences of GABAergic inhibition of dendritic spines at puberty, G6431169643, Imaging Functional Connectivity in Visual Cortex, Functional connectomics of the neocortical microcircuit, G7255690952, G7448163542, Two-photon optical control of astrocytic function
OpenAlex ID: W2275242227