Year: 2007
Type: article
Abstract: Lon ProteaseAbstract 4. 23 The initial and final steady-state rates for human Lon peptide hydrolysis vary during inhibition by 4 4. 24 The peptidyl boronate 4 inhibits human Lon peptide hydrolysis via a twostep mechanism 4.25 Proposed mechanism for peptidyl boronate inhibition of serine and threonine proteases 4.26 Reaction pathway for inhibition of Lon by peptidyl boronates 5.1 Amino acid sequence of human StAR 5.2 Summary of the majority of known StAR mutations found in patients with lipoid CAH 5.3 N-terminal of recombinant StAR 5.4 Coomassie stain of purified recombinant human StAR 5.5 Structure of NBD-cholesterol 5.6 Fluorescent detection of StAR binding to NBD-cholesterol 5.7 Increased fluorescent signal from StAR binding to NBD-cholesterol is concentration dependent 5.8 Representative binding isotherm for StAR binding to NBD-cholesterol 5.9 In vitro phosphorylation of human StAR by protein kinase A 5.10 Immunoblot analysis of human Lon degradation of human StAR 5.11 Structure of MLN64 D.1 HPLC trace of the fluorescent analog of compound 1 D.2 HPLC trace of the non-fluorescent analog of compound 1 D.3 HPLC trace of the fluorescent analog of compound 2 D.4 HPLC trace of the non-fluorescent analog of compound 2 D.5 HPLC trace of compound 3 D.6 HPLC trace of compound 4 D.7 HPLC trace of compound 5 D.8 HPLC trace of compound 6 D.9 HPLC trace of compound 7 D.10 HPLC trace of compound 8 D.11 HPLC trace of compound 9 D.12 HPLC trace of compound 10 D.13 HPLC trace of compound 11 D.14 HPLC trace of compound 12 D.15 HPLC trace of ZL 3 OH E.1 MALDI mass spectrum of the fluorescent analog of compound 1 E.2 Positive mode electrospray ionization mass spectrum of the fluorescent analog of compound 2 E.3 Positive mode electrospray ionization mass spectrum of the nonfluorescent analog of compound 2 E.4 Positive mode electrospray ionization mass spectrum of compound 3 E.5 Positive mode electrospray ionization mass spectrum of compound 4 E.6 Positive mode electrospray ionization mass spectrum of compound 5 E.7 Positive mode electrospray ionization mass spectrum of compound 6 E.8 Positive mode electrospray ionization mass spectrum of compound 7 E.9 Positive mode electrospray ionization mass spectrum of compound 8 E.10 Positive mode electrospray ionization mass spectrum of compound 9 E.11 Positive mode electrospray ionization mass spectrum of compound 10 E.12 Positive mode electrospray ionization mass spectrum of compound 11 E.13 Positive mode electrospray ionization mass spectrum of compound 12 E.14 Positive mode electrospray ionization mass spectrum of ZL 3 OH F.1 S. Typhimurium Lon degradation of the fluorescent analog of 1 F.2 S. Typhimurium Lon degradation of the non-fluorescent analog of 1 F.3 S. Typhimurium Lon degradation of the fluorescent analog of 2 F.4 S. Typhimurium Lon degradation of the non-fluorescent analog of 2 F.5 Human Lon degradation of the fluorescent analog of 1 F.6 Human Lon degradation of the non-fluorescent analog of 1 F.7 Human Lon degradation of the fluorescent analog of 2 F.8 Human Lon degradation of the non-fluorescent analog of 2 H.1 Limited tryptic digest of human Lon H.2 Protein sequencing of trypsin protected protein fragments CHAPTER 2 Purification and Steady-State Kinetic Characterization of the Human and Salmonella enterica serovar Typhimurium Lon Protease **
Author: Hilary Frase
Institution: Case Western Reserve University
Language: English
Topic: Heat shock proteins research
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2276428114