Work

Imaging Kinase Activity at Protein Scaffolds

Year: 2013

Type: book-chapter

Abstract: Kinase signaling is under tight spatiotemporal control, with signaling hubs within the cell often coordinated by protein scaffolds. Genetically encoded kinase activity reporters afford a unique tool to interrogate the rate, amplitude, and duration of kinase signaling at specific locations throughout the cell. This protocol describes how to assay kinase activity at a protein scaffold in live cells using a fluorescence resonance energy transfer (FRET)-based kinase activity sensor for protein kinase D (PKD) as an example.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-622-1_10

Authors: Maya T. Kunkel, Alexandra C. Newton

Institution: University of California San Diego

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: G2305413833, PKA & PKC Targeting Mechanisms (Program Project), Structure, Function and Regulation of Protein Kinase C