An Actinomycetes Producing Anticandida Isolated from Cajuput Rhizosphere: Partial Identification of Isolates and Amplification of pks-I genes
Year: 2010
Type: article
Abstract: : Actinomycetes have been the most prolific producer of various kinds of antifungal metabolites, and \nmany of them are described as being produced by polyketide synthetases (pks). We present strain of \nActinomycetes producing anticandida isolated from rhizosphere plant for amplification of PKS-I genes. The \nisolate was obtained from Wanagama I Forest UGM Yogyakarta. Gene of seven isolates, from total of 173 \nisolates, were amplified using degenerate primer to detect the presence of PKS genes. One strain that is \nnamed Streptomyces sp. GMR-22 was partialy identified as anticandida producing actinomycete. The strain \nshown the strongest activity against Candida albicans. Based on bioautography assay, one spot active with Rf \n0.57 was appeared as bright yellow by cerrium sulphate but it was and not visible on UV and lights.
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $150
OpenAlex ID: W2277389112