Year: 2000
Type: dissertation
Abstract: ABSTRACTThe ionotropic glutamate receptors (iGluRs) are postsynaptic ion channelsinvolved in excitatory neurotransmission. The iGluRs can be classified according totheir specific agonists into N-methyl- D -aspartate (NMDA), α -amino-5-hydroxy-3-methyl-4-isoxazole propionic acid (AMPA) and kainate receptors. Each of these classescontains several homologous subunits that assemble in a subtype-specific mannerinto oligomeric (tetra/pentameric) complexes.The iGluRs are integral membrane proteins for which there is as yet no structureavailable. However, some structural features of the subunits, including the primarystructure and its modifications, topology and domain organisation, have been solved.Extensive biochemical and biophysical characterisation has been so far hampered bythe lack of sufficient amounts of homogeneous material.In this study, the production and purification of an AMPA-type glutamatereceptor was investigated. Recombinant GluRD receptors were expressed inSpodoptera frugiperda Sf21 insect cells and purified by affinity chromatography.The purified receptor preparation contained over 2000 pmol of high-affinity (K
Source: Työväentutkimus Vuosikirja
Author: Arja Kuusinen
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
Funder: Helsingin Yliopisto
OpenAlex ID: W2277939314