Work

Mice lacking the thrombin receptor, PAR1, have normal skin wound healing.

Year: 1997

Type: article

Abstract: Thrombin's actions on platelets, macrophages, fibroblasts, and endothelial cells have prompted the hypothesis that thrombin may be important for inflammatory and fibroproliferative processes in wound healing. Protease-activated receptor 1 (PAR1) is a G-protein-coupled receptor that mediates many of the cellular activities of thrombin. To test the role of this receptor in vivo, we generated PAR1-deficient mice. Despite the observation that fibroblasts cultured from these mice lacked responsiveness to thrombin in vitro, we now report that there was no difference detected between wild-type and PAR1-deficient mice in skin wound healing assays including time to closure of open wounds, tensile strength of healed incisional wounds, wound histology, and hydroxyproline/DNA content of wound implants. We conclude that PAR1 is not necessary for normal skin wound healing in mice.

Source: Europe PMC (PubMed Central)

Authors: A J Connolly, D Y Suh, T K Hunt, S R Coughlin

Institution: University of California, San Francisco

Language: English

Topic: Wound Healing and Treatments

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: A STUDY OF WOUND HEALING, INFLAMMATION AND INFECTION, MURINE MODELS TO INVESTIGATE THE HEMATOLOGIC SYSTEM, TRANSGENIC MODELS OF THROMBIN RECEPTOR FUNCTION