Work

Emerging New Strategies for Successful Metabolite Identification in Metabolomics

Year: 2016

Type: review

Abstract: This review discusses strategies for the identification of metabolites in complex biological mixtures, as encountered in metabolomics, which have emerged in the recent past. These include NMR database-assisted approaches for the identification of commonly known metabolites as well as novel combinations of NMR and MS analysis methods for the identification of unknown metabolites. The use of certain chemical additives to the NMR tube can permit identification of metabolites with specific physical chemical properties.

Source: Bioanalysis

DOI: 10.4155/bio-2015-0004

Authors: Kerem Bingol, Lei Bruschweiler-Li, Dawei Li, Bo Zhang, Mouzhe Xie, Rafael Brüschweiler

Institutions: Pacific Northwest National Laboratory, The Ohio State University

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: Southeast Resource Center for Integrated Metabolomics (SECIM), G9000290167, G969397630