Effects of Human Hair Keratin Alkylation on Growth Factor Delivery and Cellular Response: Implications in Bone Regeneration
Year: 2014
Type: article
Abstract: by Sangheon Han Bone injury due to congenital defects or injury is a clinical challenge.Keratins are structural intermediate proteins that can be obtained from multiple sources including human hair.Keratins are characterized by a high cysteine residue content in their primary structure, leading to the ability to form covalent disulfide crosslinks.This inherent cysteine content provides a chemical mechanism by which to control the degradation rate of keratin hydrogels: modulation of disulfide crosslinking.This thesis work seeks to understand cellular and bone morphogenetic protein 2 (BMP-2) interaction with keratins modified with alkyl groups at cysteine residues to achieve different degrees of disulfide crosslinking.Insulin-like growth factor 1 (IGF-1) and ciprofloxacin were also examined to better understand the delivery mechanism of drugs from these alkylated keratin biomaterials.The results of this thesis provide a better understanding of the mechanisms by which keratin hydrogels may serve as a platform for treatment of bone injuries.
Author: Han, Sangheon
Institution: Miami University
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
Funder: University of Miami
OpenAlex ID: W2282782153