Year: 2015
Type: dissertation
Abstract: [Abstract] The wide scale use of metal oxide nanopartic1es (NP) in the world consumer market has resulted in increase in likelihood of exposure to human beings. The potential toxic effects of these NP on mammal cells have been extensively studied. However, studies regarding neurotoxicity and specific effects on neuronal systems are very scarce. Therefore, the aim of this thesis was assessing Ihe toxic potential of titanium dioxide NP (TiO,-NP), zinc oxide NP (ZnONP) and silica-coated iron oxide NP (S-ION) on human SH-SY5Y neuronal cells, to elucidate the possible mechanism of tbeir adverse effects at tbe cellular and molecular level on the nervous system. Afier NP characterization, a battery of assays was performed to evaluate tbe viability, cytotoxicity, genotoxicity and oxidative damage in NP-exposed SH-SY5Y cells. Obtai,n ed results showed that internalisation potential of each NP was different: TiO,-NP and ION were effectively internalized in a concentration- and time-dependent manner; nevertheless, ZnO-NP were unable lo be internalized by cells. The NP potential to induce toxic effects was material-dependent: TiO,-NP did not reduce tbe viability; ION presented in general low cytotoxicity, whereas ZnO-NP affected the cells most at the cytotoxicity leve!. Similar to cytotoxicity, genotoxicity-inducing potential of metal oxide NP was found to be different for each NP. Furthermore, toxicity was found to be mediated by oxidative stress in ZnO-NP and SION exposed cells, but TiO,-NP did not induce such oxidation-related changes. The results obtained in this work contributed to increase the knowledge on tbe genotoxic and cytotoxic potential of metal oxide NP in general, and specifically on human neuronal cells.
Source: RUC (Universidade Da Coruña)
Author: Gözde Kılıç
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2283870730