Feasibility of the use of capillary electrophoresis for the study of vldl assembly intermediates
Year: 2006
Type: dissertation
Abstract: The chicken has long been a model used for the study of plasma lipoproteins due\nto the ability to increase VLDL production by administration of estrogen. In this study\nwe were able to demonstrate successful isolation of VLDL assembly intermediates from\nthe livers of hens, roosters, and estrogen treated rosters. Particle diameter of first step\nparticles, as determined by dynamic laser light scattering, was decreased from an\naverage diameter of 31.5 nm in untreated birds, to 16.1 nm 12 hours after estrogen\ntreatment. Effects of estrogen waned after 24 hours and particle diameter of first step\nparticles increased to an average of 23.9 nm. These assembly intermediates, as well as\nplasma VLDL and VLDLy, were successfully studied using capillary electrophoresis\n(CE). Effective mobilities of intact plasma VLDL and first step particles decreased after\nestrogen administration. Hen VLDL showed a single uniform peak whereas rooster\nVLDL separated into distinct “subclasses”. Delipidated VLDL, VLDLy and first step\nassembly intermediates were also successfully separated using CE. This thesis is dedicated to my family who always encouraged me through this process.
Author: Elizabeth Anne White
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2284070068