Work

The Hydrodynamic Tail Vein Assay as a Tool for the Study of Liver Promoters and Enhancers

Year: 2013

Type: book-chapter

Abstract: The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver. Here, we describe the use of this technique to test for regulatory activity of liver promoters and enhancers, using a dual luciferase reporter system as the readable/measureable output and how this application can be used for pharmacogenomic studies.

Source: Methods in molecular biology

DOI: 10.1007/978-1-62703-435-7_18

Authors: Mee J. Kim, Nadav Ahituv

Institution: University of California, San Francisco

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Human Genome Research Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases