Work

Synthesis of activated 3′-amino-3′-deoxy-2-thio-thymidine, a superior substrate for the nonenzymatic copying of nucleic acid templates

Year: 2016

Type: article

Abstract: We present a scalable synthesis of 3'-amino-3'-deoxy-2-thio-thymidine-5'-phosphoro-2-methylimidazolide, an activated monomer that can copy adenosine residues in nucleic acid templates rapidly without a polymerase. The sulfur atom substitution enhances the rate of template copying by 5-fold compared with the 3'-amino-3'-deoxy-T monomer, while the 3'-amino monomers exhibit a 2- to 30-fold enhancement compared with their ribonucleotide counterparts.

Source: Chemical Communications

DOI: 10.1039/c5cc10317g

Authors: Enver Cagri Izgu, Seung Soo Oh, Jack W. Szostak

Institutions: Howard Hughes Medical Institute, Harvard University, Massachusetts General Hospital, Center for Computational and Integrative Biology, Massachusetts General Hospital

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Howard Hughes Medical Institute, Simons Foundation

Award: G2648730350