The corticotropin-releasing factor-like diuretic hormone 44 (DH 44 ) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster
Year: 2016
Type: article
Abstract: Malpighian tubules are critical organs for epithelial fluid transport and stress tolerance in insects, and are under neuroendocrine control by multiple neuropeptides secreted by identified neurons. Here, we demonstrate roles for CRF-like diuretic hormone 44 (DH44) and Drosophila melanogaster kinin (Drome-kinin, DK) in desiccation and starvation tolerance. Gene expression and labelled DH44 ligand binding data, as well as highly selective knockdowns and/or neuronal ablations of DH44 in neurons of the pars intercerebralis and DH44 receptor (DH44-R2) in Malpighian tubule principal cells, indicate that suppression of DH44 signalling improves desiccation tolerance of the intact fly. Drome-kinin receptor, encoded by the leucokinin receptor gene, LKR, is expressed in DH44 neurons as well as in stellate cells of the Malpighian tubules. LKR knockdown in DH44-expressing neurons reduces Malpighian tubule-specific LKR, suggesting interactions between DH44 and LK signalling pathways. Finally, although a role for DK in desiccation tolerance was not defined, we demonstrate a novel role for Malpighian tubule cell-specific LKR in starvation tolerance. Starvation increases gene expression of epithelial LKR. Also, Malpighian tubule stellate cell-specific knockdown of LKR significantly reduced starvation tolerance, demonstrating a role for neuropeptide signalling during starvation stress.
Source: Peptides
Institutions: University of Glasgow, University of Copenhagen
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,294
Funders: Wellcome Trust, European Commission, Carlsbergfondet, Danmarks Frie Forskningsfond, Stockholms Universitet, National Institutes of Health, Directorate for Biological Sciences, Horizon 2020 Framework Programme, Biotechnology and Biological Sciences Research Council, National Institute of General Medical Sciences
Awards: Drosophila Transgenic RNAi Resource Project, Functional genomics of the enigmatic stellate cell, G2364950913, G2461055997, G3324789014, G365676983, Novel biocontrol agents for insect pests from neuroendocrinology, Molecular functions in disease, Neuroendocrine control of environmental stress responses by Drosophila and insect disease vectors, G4937468798, G5570099221, G6125330555, G8858395165
OpenAlex ID: W2284531539