Synthetically Modified Viral Capsids as Versatile Carriers for Use in Antibody-Based Cell Targeting
Year: 2015
Type: article
Abstract: The present study describes an efficient and reliable method for the preparation of MS2 viral capsids that are synthetically modified with antibodies using a rapid oxidative coupling strategy. The overall protocol delivers conjugates in high yields and recoveries, requires a minimal excess of antibody to achieve modification of more than 95% of capsids, and can be completed in a short period of time. Antibody-capsid conjugates targeting extracellular receptors on human breast cancer cell lines were prepared and characterized. Notably, conjugation to the capsid did not significantly perturb the binding of the antibodies, as indicated by binding affinities similar to those obtained for the parent antibodies. An array of conjugates was synthesized with various reporters on the interior surface of the capsids to be used in cell studies, including fluorescence-based flow cytometry, confocal microscopy, and mass cytometry. The results of these studies lay the foundation for further exploration of these constructs in the context of clinically relevant applications, including drug delivery and in vivo diagnostics.
Source: Bioconjugate Chemistry
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
Funders: U.S. Department of Defense, Howard Hughes Medical Institute, Bill and Melinda Gates Foundation, W. M. Keck Foundation, California Institute for Regenerative Medicine, Northrop Grumman, European Commission, National Institutes of Health, U.S. Food and Drug Administration, Genentech, U.S. Public Health Service, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Center for Research Resources, Congressionally Directed Medical Research Programs
Awards: G1084971718, Highly multiplexed ion-beam tissue molecular imaging with sub-micron resolution, Systems Approach to Immunity and Inflammation, G1406188611, G1586610190, G2319365879, G2691172763, Modeling the Role of Differentiation in Cancer Progression, G291137109, G3449621863, G5201313865, Integrating mass cytometric and transcriptomic profiles of solid tumors, G5635937405, MicroRNAs as T cell sensitivity Rheostats, University of Virginia - to identify why oral poliovirus and rotavirus vaccines are less effective in the developing world, in order to develop new approaches to protect ch..., G7117345307, G7122446920, Acquisition of a QTOF Mass Spectrometer, (PQD2)New Biomarkers and Pathways to Enhance Cure in Ovarian Cancers, Understanding Influenza and SARS-CoV-2 vaccine responses, in vivo and in vitro, Therapeutic Opportunities To Target Tumor Initiating Cells in Solid Tumors
OpenAlex ID: W2284921669