Optimization of low serum human umbilical cord matrix mesenchymal stem cell (umcMsC) culture for conditioned media preparation: in vitro and in vivo wound healing applications
Year: 2011
Type: dissertation
Abstract: Research in the area of mesenchymal stem cells (MSCs) has come to prove the extensive therapeutic value of these cells. Increasing evidence supports that MSCs play a role in tissue regeneration not by means of cellular engraftment and differentiation, but through the secretion of soluble trophic factors which enhance and assist in the repair and regeneration of damaged tissues by paracrine activation of surrounding cells. This thesis aims to evaluate the therapeutic value of conditioned media prepared from umbilical cord matrix derived MSC (ucmMSC) culture on cellular migration and wound regeneration. For this purpose an in vitro migrational scratch assay in human dermal fibroblasts and in human keratinocytes; and an in vivo wound healing assay in the mouse model were performed. A novel ovine model for the wound healing assay was also developed. Conditioned media containing 0% and 2% FBS were prepared with confluent or growing ucmMSCs cultures and growing bmMSC and applied to the models mentioned above. Media supplemented with 0% and 2% FBS that were not in contact with cells were herein used as controls. Overall, it could be observed that conditioned media enhanced cellular migration when compared to the respective controls. Moreover, it was concluded that growing MSCs secreted a richer protein profile which resulted in increased cellular migration and consequent wound healing, further validating the therapeutic value of MSCs in cutaneous wound healing via secretion of soluble trophic factors. Since the use of ucmMSCs represents an inexhaustible source of easily isolated MSCs from a non-controversial origin, future mass production of MSC trophic factors could be achieved in an off-the-shelf manner. The prospective of a commercially available concentrate of MSC secreted factors which accelerates the healing of cutaneous injuries upon topical application would be a momentous achievement, reducing the invalidity and morbidity associated with cutaneous wounding and excessive scarring.
Author: Elysse C. Filipe
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2285488396