Year: 2005
Type: article
Abstract: The purpose of this study was to identify pancreatic proteins that are involved in the development of type 2 diabetes mellitus (T2DM). C57BL/6J male mice fed a high-fat diet become obese and develop T2DM, which serve as models for the disease in humans. The pancreas is important in producing hormones that regulate blood glucose and it has been postulated to be a key organ in the etiology of T2DM. In this study, proteins were extracted from the pancreas of diabetic and control mice and were resolved by a procedure termed 2 dimensional gel electrophoresis (2-DE). The pancreatic protein profiles were compared between the control and diabetic mice. Differentially expressed protein spots were identified by mass spectrometry analysis. Among 11 differential protein spots detected, the protein levels of REG1 (3 spots) and REG2 (1 spot) were higher in diabetic mice when compared with controls. Reg1 has been shown to be involved in the regeneration of pancreatic β-cells. The up-regulation of Reg1 and Reg2 in diabetic mice may reflect the effort of the pancreas to ameliorate the hyperglycemic condition by stimulating the proliferation of pancreatic β-cells, therefore, enhancing insulin secretion. Rho GDP-dissociation inhibitor 1 (GDI-1), 1-Cys peroxiredoxin protein, and pancreatic elastase 3B were also up-regulated in diabetic mice relative to controls. Glutathione peroxidase, a GSH-dependent enzyme to remove hydrogen peroxide and fatty acid hydroperoxide and the most abundant isoform in the glutathione peroxidase gene family, was found to be down-regulated in the diabetic mice at later stages. The down-regulation of glutathione peroxidase together with over-production of reactive oxidative species in the pancreas of diabetic mice could contribute to the 94 progressive deterioration of β-cell function. Also, the protein levels of the receptor of activated protein kinase C1 were decreased in diabetic mice when compared with normal controls.
Author: Linghua Qiu
Institution: Ohio University
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2287422692