Effects of Growth Hormone, IGF-1, or Combination Therapy on Muscle Fiber Type Composition in Diabetic Mice
Year: 2011
Type: article
Abstract: Introduction: Type 2 diabetes mellitus (T2DM) is a growing health problem.Despite inducing insulin resistance in many circumstances, growth hormone (GH) has been studied as a treatment for T2DM in both humans and mice.Many of the effects of GH are mediated via insulin-like growth factor-1 (IGF-1).Skeletal muscle is an important mediator of insulin resistance and blood glucose uptake.GH may cause a shift toward a slower fiber type profile in skeletal muscle, which may be associated with greater glucose uptake.Overall Goal: To examine the effects of administration of GH, IGF-1, or both on the muscle fiber type profile and muscle fiber cross-sectional area in diabetic mice.Methods: C57BL/6J mice were split into five groups as follows: Control (low-fat diet + PBS; n=10), DM (high-fat diet + PBS; n=10), GH (high-fat diet + GH; n=10), IGF-1 (high-fat diet + IGF-1; n=10), Combo (high-fat diet + GH and IGF-1; n=9).Mice were on their respective diets for 16 weeks followed by 3 weeks of hormone or PBS injections.The high-fat diet has previously been shown to induce type 2 diabetes.At the end of the injection period, the mice were sacrificed and the triceps surae muscle group (soleus, plantaris, and gastrocnemius muscles) was removed and weighed.Muscle fiber type Abstract ......
Author: Sean Schumm
Institution: Ohio University
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2287428124