Work

Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype

Year: 2016

Type: article

Abstract: A Micropallet-Array-based strategy allowing the identification of cells of defined phenotype in complex mixtures, such as would be obtained from a tissue biopsy, is presented. Following the distribution of single adherent cells from the mixture on individual pedestals, termed "micropallets", immunofluorescent confocal imaging is applied to interrogate the expression of five cell surface molecules.

Source: Advanced Healthcare Materials

DOI: 10.1002/adhm.201500859

Authors: Trisha M. Westerhof, Guann‐Pyng Li, Mark Bachman, Edward L. Nelson

Institutions: University of California, Irvine, University of California, Irvine Medical Center

Language: English

Topic: Cell Image Analysis Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, Congressionally Directed Medical Research Programs