The establishment of in vitro culture and genetic transformation of the wheat Glu-1Dx5 gene to rice plants by gene gun bombardment
Year: 2007
Type: dissertation
Abstract: Transient expression of synthetic green fluorescent protein (sgfp) gene mediated byAgrobacterium is rapid and useful approach for visual monitoring the genetic transformation event in transformed cells/tissues of examined genotype.The significant differences in transient expression of two genotypes were found with regard to the osmotic treatment (0.4 M mannitol), solid-subcultured callus, and 10 min.air drying.While, no significant differences in sgfp expression were observed in two genotypes on without air-drying and 5 min.air-drying of calluses prior immersed in Agrobacterium suspension.Surprisingly, the sgfp expression could not be detected in suspension-subcultured callus of both cultivars.The highest sgfp expression was achieved in Nipponbare callus treated with 10 min.air drying.The level of green fluorescent spots was higher in Nipponbare than that in Fatmawati, however, plants regenerated from Fatmawati were considerably comparable with those of Nipponbare.Seventeen and 16 transgenic rice plants expressing sgfp transgene were obtained from Nipponbare and Fatmawati, respectively.
Author: Nono Carsono
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2290431716